翻訳と辞書 Words near each other ・ Photosynthetic pigment ・ Photosynthetic reaction centre ・ Photosynthetic reaction centre protein family ・ Photosynthetic state transition ・ Photosynthetically active radiation ・ Photosystem ・ Photosystem I ・ Photosystem II ・ Photosystem II light-harvesting protein ・ Phototaxis ・ Phototendering ・ Phototheca ・ Phototherapeutic keratectomy ・ Photothermal effect ・ Photothermal microspectroscopy ・ Photothermal optical microscopy ・ Photothermal spectroscopy ・ Photothermal therapy ・ Photothèque (Luxembourg) ・ PhotoToMovie ・ Phototool ・ Phototoxic tar dermatitis ・ Phototoxicity ・ Phototoxin ・ Phototrope ・ Phototroph ・ Phototrophic ・ Phototrophic biofilms ・ Phototrophic prokaryotes ・ Phototropin Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Photothermal optical microscopy ： ウィキペディア英語版 Photothermal optical microscopy Photothermal optical microscopy / "photothermal single particle microscopy" is a technique that is based on detection of non-fluorescent labels. It relies on absorption properties of labels (gold nanoparticles, semiconductor nanocrystals, etc.), and can be realized on a conventional microscope using a resonant modulated heating beam, non-resonant probe beam and lock-in detection of photothermal signals from a single nanoparticle. It is the extension of the macroscopic photothermal spectroscopy to the nanoscopic domain. The high sensitivity and selectivity of photothermal microscopy allows even the detection of single molecules by their absorption. Similar to Fluorescence Correlation Spectroscopy (FCS), the photothermal signal may be recorded with respect to time to study the diffusion and advection characteristics of absorbing nanoparticles in a solution. This technique is called photothermal correlation spectroscopy (PhoCS). == Forward detection scheme == In this detection scheme a conventional scanning sample or laser-scanning transmission microscope is employed. Both, the heating and the probing laser beam are coaxially aligned and superimposed using a dichroic mirror. Both beams are focused onto a sample, typically via a high-NA illumination microscope objective, and recollected using a detection microscope objective. The thereby collimated transmitted beam is then imaged onto a photodiode after filtering out the heating beam. The photothermal signal is then the change $\backslash Delta$ in the transmitted probe beam power $P\_d$ due to the heating laser. To increase the signal-to-noise ratio a lock-in technique may be used. To this end, the heating laser beam is modulated at a high frequency of the order of MHz and the detected probe beam power is then demodulated on the same frequency. For quantitative measurements, the photothermal signal may be normalized to the background detected power $P\_$ (which is typically much larger than the change $\backslash Delta\; P\_d$), thereby defining the relative photothermal signal $\backslash Phi$ $$ \Phi=\frac\right)-P_d\left(\text\right)} 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Photothermal optical microscopy」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.